|
KMID : 0603820070130020135
|
|
Journal of Experimental & Biomedical Science
2007 Volume.13 No. 2 p.135 ~ p.140
|
|
|
Effects of Intravenous Administration of Taurocholic Acid on Hepatic Catechol-O-Methyltransferase Activity in Rats with Choledocho-Caval Shunt
|
|
Do Jun-Young
Mun Kyo-Cheol
Kim You-Hee
Kwak Chun-Sik
|
|
|
Abstract
|
|
|
|
The possible mechanism of decreased catechol-O-methyltransferase (COMT) activity in cholestatic rat liver was studied. Hepatic and serum COMT activities were determined from the experimental rats with choledocho-caval shunt (CCS). The Michaelis-Menten constants in this hepatic enzyme was also measured. The activities of cytosolic, mitochondrial and mircosomal COMT as well as their V_{max} values were found to be decreased significantly in CCS plus taurocholic acid (TCA) injected group than in the control group, such as CCS alone groups. However, their K_m values in the experimental groups did not vary. Seru4m COMT activity increased slightly in the CCS plus TCA injerted group than in the control group. The above results suggest that TCA represses biosynthesis of the COMT in the liver, The elevated activity of the serum COMT is believed to be caused by the increment of membrane permeability of hepatocytes upon TCA mediated liver cell necrosis.
|
|
|
KEYWORD
|
|
|
Catecho1-O-methyltransferase, Choledocho-caval shunt, Taurocholic acid
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|